Journal: bioRxiv

Article Title: Analysis of Serologic Cross-Reactivity Between Common Human Coronaviruses and SARS-CoV-2 Using Coronavirus Antigen Microarray

doi: 10.1101/2020.03.24.006544

Figure Lengend Snippet: Non-coronavirus respiratory virus antigens on microarray.

Article Snippet: hMPV , B , PER/CFI0466/2010/B , G , , HEK293 , Sino Biological , 52D-238S-His , Custom.

Techniques: Microarray, Expressing, Construct

Journal: bioRxiv

Article Title: Analysis of Serologic Cross-Reactivity Between Common Human Coronaviruses and SARS-CoV-2 Using Coronavirus Antigen Microarray

doi: 10.1101/2020.03.24.006544

Figure Lengend Snippet: Coronavirus antigens on microarray.

Article Snippet: hMPV , B , PER/CFI0466/2010/B , G , , HEK293 , Sino Biological , 52D-238S-His , Custom.

Techniques: Microarray, Expressing, Construct

Journal: Molecular Cancer

Article Title: Pregnane X receptor is associated with unfavorable survival and induces chemotherapeutic resistance by transcriptional activating multidrug resistance-related protein 3 in colorectal cancer

doi: 10.1186/s12943-017-0641-8

Figure Lengend Snippet: PXR positively correlates with MRP3 expression in colorectal cancer. a Immunohistochemical analysis of PXR and MRP3 expression was performed on cancerous and adjacent noncancerous samples. Low levels of PXR and MRP3 expression were observed in noncancerous tissues compared with cancerous tissues. Scale bars represent 50 μm. Representative samples were shown. b PXR and MRP3 expression levels were analyzed in cancerous and adjacent noncancerous tissues. PXR and MRP3 expression was higher in cancerous tissues than in adjacent noncancerous tissues. The error bars indicate the s.d. c A significant positive correlation between PXR and MRP3 expression was detected in colorectal cancer. The scale bars represent 50 μm. Representative samples were shown. d A significant positive correlation between PXR and MRP3 expression was also observed in two other independent colorectal cancer cohorts from the TCGA database. Heat map was generated to compare PXR and MRP3 expression in the TCGA colorectal cancer gene expression dataset ( https://genome-cancer.soe.ucsc.edu/proj/site/xena/heatmap/ )

Article Snippet: The MRP3 siRNA was purchased from Santa Cruz Biotechnology. b Analysis of the effects of L-OHP on cancer cell proliferation when different PXR and MRP3 expression.

Techniques: Expressing, Immunohistochemical staining, Generated, Gene Expression

Journal: Molecular Cancer

Article Title: Pregnane X receptor is associated with unfavorable survival and induces chemotherapeutic resistance by transcriptional activating multidrug resistance-related protein 3 in colorectal cancer

doi: 10.1186/s12943-017-0641-8

Figure Lengend Snippet: PXR activates MRP3 transcription by directly binding to its promoter. a Ectopic expression of PXR significantly enhances the endogenous expression of MRP3. PXR and MRP3 expressions were examined by RT-PCR and WB, and β-actin was used as an internal control. b Different regions of the MRP3 promoter were cloned into a luciferase reporter construct. c , d PXR targets MRP3 directly, and the key PXR binding region is located between -884 to -620bp of the MRP3 promoter as determined by luciferase reporter assays. The results were normalized to internal controls from three experiments. e ChIP-PCR was performed to identify MRP3 as a PXR target. Jaspar and ConSite predicted the PXR binding sites as located between -796 to -782bp of the MRP3 promoter. f The key PXR binding region is located between -884 to -754bp of the MRP3 promoter determined by luciferase reporter assays. The results were normalized to internal controls from three experiments. ** P < 0.01. g The effects of PXR on wild-type and mutated/deleted MRP3 promoter (between -796 to -782bp) activity. Error bars indicate s.d. ( n = 3), *, mutated sites; - , deleted sites. ** P < 0.01

Article Snippet: The MRP3 siRNA was purchased from Santa Cruz Biotechnology. b Analysis of the effects of L-OHP on cancer cell proliferation when different PXR and MRP3 expression.

Techniques: Binding Assay, Expressing, Reverse Transcription Polymerase Chain Reaction, Control, Clone Assay, Luciferase, Construct, Activity Assay

Journal: Molecular Cancer

Article Title: Pregnane X receptor is associated with unfavorable survival and induces chemotherapeutic resistance by transcriptional activating multidrug resistance-related protein 3 in colorectal cancer

doi: 10.1186/s12943-017-0641-8

Figure Lengend Snippet: MRP3 is a functionally target gene of PXR in drug resistance. a The expression of PXR and MRP3 was analyzed by WB at 48h after transfecting with MRP3 small interfering RNA (siRNA). The MRP3 siRNA was purchased from Santa Cruz Biotechnology. b Analysis of the effects of L-OHP on cancer cell proliferation when different PXR and MRP3 expression. Cell numbers were counted every day after treatment for 4 days. c Analysis of the effects of L-OHP on cancer cell apoptosis when different PXR and MRP3 expression. d The transport and uptake of L-OHP in tumour cells with different PXR and MRP3 expression were assessed by mass cytometry

Article Snippet: The MRP3 siRNA was purchased from Santa Cruz Biotechnology. b Analysis of the effects of L-OHP on cancer cell proliferation when different PXR and MRP3 expression.

Techniques: Expressing, Small Interfering RNA, Mass Cytometry

Journal: Molecular Cancer

Article Title: Pregnane X receptor is associated with unfavorable survival and induces chemotherapeutic resistance by transcriptional activating multidrug resistance-related protein 3 in colorectal cancer

doi: 10.1186/s12943-017-0641-8

Figure Lengend Snippet: A schematic diagram for the mechanisms underlying PXR-regulated MRP3-mediated resistance. PXR enhances the resistance of tumor cells to the chemotherapeutic agent L-OHP via direct transcriptional activating MRP3 expression, increasing the L-OHP efflux capacity of tumor cells in CRC

Article Snippet: The MRP3 siRNA was purchased from Santa Cruz Biotechnology. b Analysis of the effects of L-OHP on cancer cell proliferation when different PXR and MRP3 expression.

Techniques: Expressing